Broadening the Scope of Immunology to Improve Human Health
In May, as series of extraordinary Immunology lectures were given at the Joseph B. Martin Conference Center which highlighted bold new directions in immunology research. On May 4 and 7, the 2012 HMS Dunham Lectures were delivered by renowned immunologist Mark Davis of Stanford University, and on May 8, a symposium titled “Breaching the Borders”, which was organized by the Program in Immunology featured talks by 14 different investigators, organized into four sections, each focused on the interface of immunology with other areas of science and medicine.
The Dunham Lectures
The Dunham lectureship was established in 1923 by Mary Dows Dunham in memory of her late husband, Edward Dunham. Of the 75 medical researchers honored with a Dunham lectureship, 28 have been Nobel laureates.
Mark Davis, the 2012 Dunham lecturer, is the Burt and Marion Avery Family Professor of Immunology at the Stanford University School of Medicine and director of the Stanford Institute for Immunology, Transplantation and Infection. Davis is widely known for identifying a number of important genes essential for immunological function, such as elusive T-Cell receptor genes, which his lab discovered in the 1980s. He has also helped pioneer diagnostic assays for immune function, including a high throughput cellular array system, which can obtain information from a variety of types of blood cells simultaneously.
“Davis has done pioneering work to help us understand the immune system from a broad perspective,” said William Chin, HMS executive dean for research. “Anyone in our community with an interest in immunology and related areas should be excited about these lectures.”
Davis's lectures focused on two distinct areas of research: a broad-based effort to use “humans in the wild” as a model organism for understanding the immune system that he is leading through Stanford's Human Immune Monitoring Center, and a continuation of his decades of work examining the fundamental operating dynamics of T-cells, immune cells which function as sentinels and switchboards that detect threats within the body and orchestrate responses to them.
How healthy is your immune system?
In the first of his two lectures, Davis focused on human immunity. He noted that it is crucial to understand the metrics of health in a functioning human immune system.
According to Davis, the immune system is important for health, but we have had no way of measuring how robust any given individual's immune system is. For example, why do some people who receive flu vaccine remain healthy while others get the flu?
“Before you can understand disease you need to understand how the healthy organism works,” he said.
Davis is known for his pioneering work developing these techniques, including a high throughput cellular array system, which can obtain information from a variety of types of blood cells simultaneously.
In one study, Davis's team searched blood-bank blood for a complete panel of known antibodies. Hidden like needles in a haystack, they found anti-HIV memory cells in people who were HIV negative, and antibodies for bird flu in people who have never had the bird flu.
Previously it was thought that the body built these defense cells from scratch when a new danger was detected, but Davis's team has shown that the body has a complete (though very thin) wall of defenses for every possible antigen.
These defense cells were extremely rare, but the fact that they exist, he said, suggests that evolution has favored prepositioned diversity, providing some measure of defense against every possible infectious agent.
In other work, Davis's team is beginning to identify characteristics – at the biochemical level – that predict how effective a given individual's immune system is at protecting against a virus like the flu.
This broad-spectrum, curiosity-driven approach is fruitful thanks to carefully constructed collaborations that enable diverse labs to share technology, clinical records, blood samples, and varying perspectives and techniques for doing science. It is also successful because of technological breakthroughs in the last decade, such as high-resolution single-molecule imaging, and high-speed, high fidelity sequencing that allows researchers to sort through massive numbers of variations in proteins and peptides.
Structures for sensing sickness
Davis's second lecture focused on the molecular dynamics of how T-cells recognize potential pathogens and interact with other cells. New imaging techniques have enabled Davis to begin to develop a clearer picture of how T-cells sense the world around them and interact with other cells.
“The immune system has a language – how it communicates with itself and orchestrates responses – and we're just beginning to understand it,” Davis said.
One important next step will be learning more about the shape and structure of the plasma membrane, the place where T-cells meet and attempt to identify potential pathogens. By labeling specific molecules and capturing images of their motion, Davis is beginning to see how T-cells enter into dialog with the body and the world of potential attackers within.
One thing researchers have seen is that T-cell receptors are sensitive enough to detect and respond to a single instance of an antigen peptide, the same way the retina can detect a single photon. Most intra-cellular communications – say with hormones – require much heavier volume for successful signaling, Davis said, which suggests that the immune system is like a sixth sense, designed to probe the workings of the microscopic interior environment of the organism.
“You don't see that kind of sensitivity outside of the sensory apparatus,” he said.
The structures that this work is revealing are important to many areas of biology outside of immunology, Davis said and are as surprisingly new as they are crucial to the function of life.
“One of the things I love about biology,” said Davis, “is that we know so little that if you go into a particular new situation with new tools and techniques, you always find something new and exciting.”
Breaching the Borders
The discussion of groundbreaking techniques and exciting new cross-discipline collaborations in immunology continued May 8 at “Breaching the Borders,” a symposium sponsored by the HMS Division of Immunology and Department of Microbiology and Immnuobiology. Talks highlighted efforts to understand immunology and its many related systems and processes as crucially interrelated parts of the whole organism.
“This symposium [showcased] the broad range of the immunology field, as well as its fusion with other disciplines. It was inspiring to see the great number of people, especially non-immunologists, interested in hearing about how immunological processes are important in other realms, such as neurobiology and metabolism," said Diane Mathis, Morton Grove-Rasmussen Professor of Immunohematology, who organized the event.
The panels focused on NeuroImmunology, ImmunoMetabolism, the Inflammation/Cancer Connection, and Systems Immunology. One consistent theme was the overarching complexity of these tightly interconnected systems. To deal with this complexity, speakers noted that researchers need to better understand the evolutionary and development history of the immune system, and noted that new tools from bioinformatics, physics and other disciplines are providing new ways to understand that complexity.
Other participants noted that the symposium brought together diverse and often distinct communities.
“The neuroscience and immunology communities have largely existed in independent parallel universes at HMS. This was a chance to come together to confront the growing realization that the biology is intimately intertwined,” said Clifford Woolf, HMS professor of neurology and neurobiology and director of the F.M. Kirby Center and Program in Neurobiology at Children's Hospital Boston.
“Hopefully this will result in a major push to a more coherent and active neuroimmunology presence here, characterized by active collaboration and great science,” Woolf said.
“Immunology plays central roles in so many disease processes, it was great to hear about and discuss many examples,” said Steven Shoelson, HMS professor of medicine at Brigham and Women's Hospital and the Joslin Diabetes Center. “Cross fertilization between scientific disciplines promotes discovery.”
The topics and speakers at the "Breaching the Borders" symposium included:
Clifford Woolf, Ph.D., M.B.,B.Ch., Professor of Neurology, Harvard Medical School
Kevin Tracey, M.D., President, The Feinstein Institute for Medical Research
Beth Stevens, Ph.D. ,Assistant Professor of Neurology, Harvard Medical School
Vijay Kuchroo, Ph. D., Samuel L. Wasserstrom Professor of Neurology, Harvard Medical School
Anthony Ferrante, M.D., Ph.D., Dorothy & Daniel Silberberg Associate Professor of Medicine, Columbia University
Steven Shoelson, M.D., Ph.D., Professor of Medicine, Harvard Medical School
Diane Mathis, Ph.D., Morton Grove-Rasmussen Professor of Immunohematology, Harvard Medical School
Ruslan Medzhitov, Ph.D., David W. Wallace Professor of Immunobiology Yale School of Medicine, Investigator, Howard Hughes Medical Institute
Mikael Pittet, Ph.D., Assistant Professor of Radiology, Harvard Medical School
Wendy Garrett, M.D., Ph.D., Assistant Professor of Medicine, Harvard Medical School
Dimitrios Iliopoulos, Ph.D., Assistant Professor of Microbiology and Immunobiology, Harvard Medical School
Ronald Germain, M.D., Ph.D., Chief, Laboratory of Systems Biology, NIH - NIAID
Nir Hachohen, Ph.D., Assistant Professor of Medicine, Harvard Medical School, Senior Associate Member, Broad Institute
Christophe Benoist, M.D., Ph.D., Morton Grove-Rasmussen Professor of Immunohematology, Harvard Medical School