Welcome to the HMS Immunology Flow Cytometry Core Facility
NOTICE: For appointments 7-1-22 or later, please use the new PPMS scheduler here: https://ppms.us/harvard-hms/login/ and choose Flow Core from the list at top right-hand corner.
The LSR-II and Symphony instruments are available for analysis, for trained investigators.
The Aria 594 is available for self-sorting for trained users.
Operator-assisted sorting (MoFlo Astrios and Aria 561) is available Monday-Friday and every other Saturday*.
*For Saturday operator-assisted sorting, please ALSO contact Jeff Nelson at the address below at the time of booking.
For all questions, training information and Saturday sorting, contact Jeffery_Nelson@hms,harvard.edu
The Department of Immunology’s Flow Cytometry Facility provides flow cytometric analysis and cell sorting services to HMS investigators and users from affiliated institutions as well as commercial/biotech labs.
The facility currently operates:
(1) LSR-II analyzer (3-laser, 12-detector) with a high throughput sampler for multiparameter flow analysis
(2) FACS-Symphony (5 laser, 30 detector) with a high throughput sampler for high parameter analysis
(3) 2 FACSAria cell sorters for high-speed cell sorting, equipped with 4 and 5 laser systems for sorting in tubes or plates, and 4-way sorting
(4) MoFlo Astrios EQ cell sorter for high-speed cell sorting, equipped with 6 lasers (405, 488, 532, 561, 592, and 640nm) and 28 detectors is housed in a biosafety cabinet and is capable of 6-way sorting
(5) Cytek Aurora Spectral Cytometer (5 laser, 40 color) for high-resolution, high parameter analysis with high throughput sampler
(6) Agilent Penteon (5 laser, 30 fluorescent detectors) with high throughput sampler for high parameter analysis
Facility staff advise and train investigators in the applications of flow cytometry, use of the instruments, and help with study design and choice of fluorochrome combinations. Sorting is primarily staff run with the possibility of “self-operation” (for highly trained and experienced users). The analyzers are operated directly by the investigators, after training and certification by facility staff.
