Location:

Room 855/856, New Research Building, 77 Avenue Louis Pasteur, Boston, MA 02115

Hours of Operation: 
  • Monday: 9am-8pm
  • Tuesday: 10am-9pm
  • Wednesday: 9am-8pm
  • Thursday: 9am-5pm
  • Friday: 9am-5pm
Booking

Please use the following link to login or request a new PPMS calendar account: https://ppms.us/harvard-hms/login/ to book time on the equipment.

Please note that cancellations must be made at least 72 hours in advance to avoid charges.

Training:

Please e-mail Chad (Chad_Araneo@hms.harvard.edu) to arrange training prior to booking all equipment with the exception of staff-run sorting on the FACSAria, MoFlo Atrios EQ and Aurora Spectral Cell Sorter.

Sort Sample Requirements:
  • Cell concentration not to exceed 10 million cells/mL 
  • Cells are to be filtered through 40-50 uM mesh prior to sorting (after final wash or staining step)
  • Cells can be brought in 12x75 mm round bottom tubes or 15 mL conical Tubes 
  • Phenol red is not recommended in cell suspension media.
  • An unstained control and a single positive control for each fluorophore used is required.
Collection Tube Requirements:
Tube Sorting
  • All collection tubes should be filled approximately ¼  with desired buffer (cell growth media, PBS, lysis buffer, etc).
  • Eppendorf tubes or 12x75 mm round bottom tubes can be used for 1, 2, 3 or 4 way sorting, 15 mL conical tubes can be used for 1 or 2 way sorting.
  • Pen-Strep is recommended in collection media.

Plate Sorting
  • Cells can be sorted directly into 6, 12, 24, 48, or 96 well plates.
  • Wells should contain appropriate buffer.
  • Please indicate plate type on the calendar at the time of sign up.

Booking calendar

Click here to book time!