Location:
Room 855/856, New Research Building, 77 Avenue Louis Pasteur, Boston, MA 02115
Hours of Operation:
- Monday: 9am-8pm
- Tuesday: 10am-9pm
- Wednesday: 9am-8pm
- Thursday: 9am-5pm
- Friday: 9am-5pm
Booking:
Please use the following link to login or request a new PPMS calendar account: https://ppms.us/harvard-hms/login/ to book time on the equipment.
Please note that cancellations must be made at least 72 hours in advance to avoid charges.
Training:
Please e-mail Chad (Chad_Araneo@hms.harvard.edu) to arrange training prior to booking all equipment with the exception of staff-run sorting on the FACSAria, MoFlo Atrios EQ and Aurora Spectral Cell Sorter.
Sort Sample Requirements:
- Cell concentration not to exceed 10 million cells/mL
- Cells are to be filtered through 40-50 uM mesh prior to sorting (after final wash or staining step)
- Cells can be brought in 12x75 mm round bottom tubes or 15 mL conical Tubes
- Phenol red is not recommended in cell suspension media.
- An unstained control and a single positive control for each fluorophore used is required.
Collection Tube Requirements:
Tube Sorting:
- All collection tubes should be filled approximately ¼ with desired buffer (cell growth media, PBS, lysis buffer, etc).
- Eppendorf tubes or 12x75 mm round bottom tubes can be used for 1, 2, 3 or 4 way sorting, 15 mL conical tubes can be used for 1 or 2 way sorting.
- Pen-Strep is recommended in collection media.
Plate Sorting:
- Cells can be sorted directly into 6, 12, 24, 48, or 96 well plates.
- Wells should contain appropriate buffer.
- Please indicate plate type on the calendar at the time of sign up.
Booking calendar
Click here to book time!