Equipment

Take a look at all our machines available for flow cytometry.

The facility currently operates: 

Analyzers
  1. LSR-II analyzer (4-laser V-B-Y-R, 13-fluorescent detectors)
  2. FACS-Symphony A1 (4 laser V-B-Y-R, 16 fluorescent detectors)
  3. FACS-Symphony A3 (5 laser UV-V-B-Y-R, 23 fluorescent detectors)
  4. FACS-Symphony A5 (5 laser UV-V-B-Y-R, 28 fluorescent detectors)
  5. Agilent Penteon (5 laser UV-V-B-Y-R, 30 fluorescent detectors)
  6. Cytek Aurora Spectral Analyzer (5 laser UV-V-B-Y-R, 64 total detectors - 40 color capable)
Sorters
  1. FACSAria for high-speed cell sorting (4 lasers V-B-O-R, 13 fluorescent detectors)
  2. FACSAria for high-speed cell sorting (5 lasers UV-V-B-Y-R, 15 fluorescent detectors)
  3. MoFlo Astrios for high-speed cell sorting, (6 lasers V-B-G-Y-O-R, 23 fluorescent detectors) and is housed in a biosafety cabinet
  4. Cytek Aurora Spectral Sorter (5 laser UV-V-B-Y-R, 64 total detectors - 40 color capable) and is housed in a biosafety cabinet

Additional equipment details

Analyzers

For self-run flow cytometery.

These machines typically require a 1-2 hour long training before new users are ready to run flow.

LSRII

    LSRII

The BD LSRII is a 4 laser system (405, 488, 561 & 637 nm) with 13 fluorescent detectors.  

Runs 5ml polystyrene tubes or a HTS unit can be attached allowing the use of various 96 or 384 well plates.  

LSRII Optical Layout.

Symphony A1

Symphony A1

The BD Symphony A1 is a 4 laser system (405, 488, 561 & 637 nm) with 16 fluorescent detectors.  

Runs 5ml polystyrene tubes or a HTS unit is attached allowing the use of various 96 or 384 well plates.  

Symphony A1 Optical Layout.

Symphony A3

The BD Symphony A3 is a 5 laser system (355, 405, 488, 561 & 637 nm) with 23 fluorescent detectors.  

Runs 5ml polystyrene tubes only. 

Symphony A3 Optical Layout

Symphony A5

Symphony A5

The BD Symphony A5 is a 5 laser system (355, 405, 488, 561 & 637 nm) with 28 fluorescent detectors.  

Runs 5ml polystyrene tubes or a HTS unit is attached allowing the use of various 96 or 384 well plates.  

Symphony A5 Optical Layout.

Penteon

Penteon

The Agilent Penteon is a 5 laser system (349, 405, 488, 561 & 637 nm) with 30 fluorescent detectors.  

An attached plate loader accommodates various plates (including deep well) and tubes (1.5 and 5ml 40 tube racks). 

Penteon Optical Layout.

Aurora

Aurora Spectral Analyzer

The Cytek Aurora is a 5 laser spectral analyzer (355, 405, 488, 561 & 640 nm) with 64 total detectors (40 color panel).  

Runs 5ml tubes or an attached plate loader accommodates various plates.  

Aurora Optical Layout.

Sorters

For staff-run or self-run cell sorting.

These machines can be booked by anyone for staff-run sorting, five days a week.

Sorter training is available for self-use by may require several training sessions.

Aria II (594)

Aria II (594)

A 4 laser system with 12 fluorescent detectors and 4 way and plate sorting capability.  Has 405 nm, 488 nm, 594 nm, and 633 nm lasers.  

For 4-way sorting, 1.5 ml or 5 ml tubes can be used while 2-way sorting can accommodate 15 ml tubes.  Multi-well plates (6, 24, 96, or 384) with index sorting is also available.  Sample and collection block can be chilled or warmed.  

Available nozzle sizes include; 70, 85, 100 and 130 µm.  Researchers who have completed BD analyzer training can be trained to self-sort on the Aria, if desired.  

Aria 594 Optical Layout. 

Aria II (561)

Aria II

A 5 laser system with 15 fluorescent detectors and 4 way and plate sorting capability.  Has 355, 405, 488, 561, and 635 lasers.  

For 4-way sorting, 1.5 ml or 5 ml tubes can be used while 2-way sorting can accommodate 15 ml tubes.  Multi-well plates (6, 24, 96, or 384) with index sorting is also available.  Sample can be chilled or warmed during the sort. 

Available nozzle sizes include; 70, 85, 100 and 130 µm.  Researchers who have completed BD analyzer training can be trained to self-sort on the Aria, if desired.  

Aria 561 Optical Layout.

Astrios

Astrios

The Beckman Coulter MoFlo Astrios EQ high speed cell sorter has 6 lasers (405 nm, 488 nm, 532 nm, 561 nm, 592 nm, & 640 nm), 23 fluorescent detectors, and can do 6-way sorting.  

It has a forward scatter PMT available for small particle detection.  

Available nozzle sizes include;  70 & 100 µm.  

BL2 capable.  

1.5ml or 5ml tubes can be used for 6-way sorting while 15ml & 50ml tubes can be used for 2-way sorting.  Multi-well plates (6, 24, 96, 384, 1536, or custom) with index sorting is also available.  Sample and collection device can be warmed or cooled.  Staff-run only.  

Astrios Optical Layout. 

Aurora CS

Aurora CS

The Cytek Aurora CS is a 5 laser spectral sorter (355, 405, 488, 561 & 640 nm) with 61 fluorescent detectors (40 color panel).  

For 6-way sorting, 1.5ml tubes can be used while 5ml tubes can be used for 4-way sorting and 15ml tubes can be used for 2-way sorting.  96-well plates with index sorting is also available.  The sample can be warmed or cooled during sorting.  

Aurora Optical Layout.

Booking calendar

Click here to book time!

PPMS