The facility currently operates:
LSRII | The BD LSRII is a 4 laser system (405, 488, 561 & 637nm) with 13 fluorescent detectors. Runs 5ml polystyrene tubes or a HTS unit can be attached allowing the use of various 96 or 384 well plates. LSRII Optical Layout. |
Symphony A1
| The BD Symphony A1 is a 4 laser system (405, 488, 561 & 637nm) with 16 fluorescent detectors. Runs 5ml polystyrene tubes or a HTS unit is attached allowing the use of various 96 or 384 well plates. Symphony A1 Optical Layout. |
Symphony A3  | The BD Symphony A3 is a 5 laser system (355, 405, 488, 561 & 637nm) with 23 fluorescent detectors. Runs 5ml polystyrene tubes only. Symphony A3 Optical Layout |
Symphony A5
| The BD Symphony A5 is a 5 laser system (355, 405, 488, 561 & 637nm) with 28 fluorescent detectors. Runs 5ml polystyrene tubes or a HTS unit is attached allowing the use of various 96 or 384 well plates. Symphony A5 Optical Layout. |
Penteon
| The Agilent Penteon is a 5 laser system (349, 405, 488, 561 & 637nm) with 30 fluorescent detectors. An attached plate loader accommodates various plates (including deep well) and tubes (1.5 and 5ml 40 tube racks). Penteon Optical Layout. |
Aurora
| The Cytek Aurora is a 5 laser spectral analyzer (355, 405, 488, 561 & 640nm) with 64 total detectors (40 color panel). Runs 5ml tubes or an attached plate loader accommodates various plates. Aurora Optical Layout. |
Aria II (594)
| A 4 laser system with 12 fluorescent detectors and 4 way and plate sorting capability. Has 405nm, 488nm, 594nm, and 633nm lasers. For 4-way sorting, 1.5ml or 5ml tubes can be used while 2-way sorting can accommodate 15ml tubes. Multi-well plates (6, 24, 96, or 384) with index sorting is also available. Sample and collection block can be chilled or warmed. Available nozzle sizes include; 70, 85, 100 and 130micron. Researchers who have completed BD analyzer training can be trained to self-sort on the Aria, if desired. Aria 594 Optical Layout. |
Aria II (561)
| A 5 laser system with 15 fluorescent detectors and 4 way and plate sorting capability. Has 355, 405, 488, 561, and 635 lasers. For 4-way sorting, 1.5ml or 5ml tubes can be used while 2-way sorting can accommodate 15ml tubes. Multi-well plates (6, 24, 96, or 384) with index sorting is also available. Sample can be chilled or warmed during the sort. Available nozzle sizes include; 70, 85, 100 and 130micron. Researchers who have completed BD analyzer training can be trained to self-sort on the Aria, if desired. Aria 561 Optical Layout. |
Astrios
| The Beckman Coulter MoFlo Astrios EQ high speed cell sorter has 6 lasers (405nm, 488nm, 532nm, 561nm, 592nm, & 640nm), 23 fluorescent detectors, and can do 6-way sorting. It has a forward scatter PMT available for small particle detection. Available nozzle sizes include; 70 & 100 micron. BL2 capable. 1.5ml or 5ml tubes can be used for 6-way sorting while 15ml & 50ml tubes can be used for 2-way sorting. Multi-well plates (6, 24, 96, 384, 1536, or custom) with index sorting is also available. Sample and collection device can be warmed or cooled. Staff-run only. Astrios Optical Layout. |
Aurora CS
| The Cytek Aurora CS is a 5 laser spectral sorter (355, 405, 488, 561 & 640nm) with 61 fluorescent detectors (40 color panel). For 6-way sorting, 1.5ml tubes can be used while 5ml tubes can be used for 4-way sorting and 15ml tubes can be used for 2-way sorting. 96-well plates with index sorting is also available. The sample can be warmed or cooled during sorting. Aurora Optical Layout. |
