The facility currently operates:

Analyzers

LSRII

    LSRII

The BD LSRII is a 4 laser system (405, 488, 561 & 637nm) with 13 fluorescent detectors.  Runs 5ml polystyrene tubes or a HTS unit can be attached allowing the use of various 96 or 384 well plates.  LSRII Optical Layout.

Symphony A1

Symphony A1

The BD Symphony A1 is a 4 laser system (405, 488, 561 & 637nm) with 16 fluorescent detectors.  Runs 5ml polystyrene tubes or a HTS unit is attached allowing the use of various 96 or 384 well plates.  Symphony A1 Optical Layout.

Symphony A5

Symphony A5

The BD Symphony A5 is a 5 laser system (355, 405, 488, 561 & 637nm) with 28 fluorescent detectors.  Runs 5ml polystyrene tubes or a HTS unit is attached allowing the use of various 96 or 384 well plates.  Symphony A5 Optical Layout.

Penteon

Penteon

The Agilent Penteon is a 5 laser system (349, 405, 488, 561 & 637nm) with 30 fluorescent detectors.  An attached plate loader accommodates various plates (including deep well) and tubes (1.5 and 5ml 40 tube racks).   Penteon Optical Layout.

Aurora

Aurora Spectral Analyzer

The Cytek Aurora is a 5 laser spectral analyzer (355, 405, 488, 561 & 640nm) with 64 total detectors (40 color panel).  Runs 5ml tubes or an attached plate loader accommodates various plates.  Aurora Optical Layout.
Sorters

Aria II (594)

Aria II (594)

A 4 laser system with 12 fluorescent detectors and 4 way and plate sorting capability.  Has 405nm, 488nm, 594nm, and 633nm lasers.  For 4-way sorting, 1.5ml or 5ml tubes can be used while 2-way sorting can accommodate 15ml tubes.  Multi-well plates (6, 24, 96, or 384) with index sorting is also available.  Sample and collection block can be chilled or warmed.  Available nozzle sizes include; 70, 85, 100 and 130micron.  Researchers who have completed BD analyzer training can be trained to self-sort on the Aria, if desired.  Aria 594 Optical Layout

Aria II (561)

Aria II

A 5 laser system with 15 fluorescent detectors and 4 way and plate sorting capability.  Has 355, 405, 488, 561, and 635 lasers.  For 4-way sorting, 1.5ml or 5ml tubes can be used while 2-way sorting can accommodate 15ml tubes.  Multi-well plates (6, 24, 96, or 384) with index sorting is also available.  Sample can be chilled or warmed during the sort. Available nozzle sizes include; 70, 85, 100 and 130micron.  Researchers who have completed BD analyzer training can be trained to self-sort on the Aria, if desired.  Aria 561 Optical Layout.

Astrios

Astrios

The Beckman Coulter MoFlo Astrios EQ high speed cell sorter has 6 lasers (405nm, 488nm, 532nm, 561nm, 592nm, & 640nm), 23 fluorescent detectors, and can do 6-way sorting.  It has a forward scatter PMT available for small particle detection.  Available nozzle sizes include;  70 & 100 micron.  BL2 capable.  1.5ml or 5ml tubes can be used for 6-way sorting while 15ml & 50ml tubes can be used for 2-way sorting.  Multi-well plates (6, 24, 96, 384, 1536, or custom) with index sorting is also available.  Sample and collection device can be warmed or cooled.  Staff-run only.  Astrios Optical Layout

Aurora CS

Aurora CS

The Cytek Aurora CS is a 5 laser spectral sorter (355, 405, 488, 561 & 640nm) with 61 fluorescent detectors (40 color panel).  For 6-way sorting, 1.5ml tubes can be used while 5ml tubes can be used for 4-way sorting and 15ml tubes can be used for 2-way sorting.  96-well plates with index sorting is also available.  The sample can be warmed or cooled during sorting.  Aurora Optical Layout.